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KMID : 0387420100210010043
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Korean Society of Oriental Neuropsychiatry
2010 Volume.21 No. 1 p.43 ~ p.57
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Investigation of Neuroprotective Effect Using Herb Medicine against Dementia related Brain Damage in Organotypic Hippocampal Slice Culture
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Chu Ching-Nai
Kim Jong-Woo
Chung Sun-Yong
Park Ji-Ho
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Abstract
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Objectives: This study was designed to assess neuroprotective effects of herb medicine against Alzhheimer’s disease related brain damage in organotypic hippocampal slice culture.
Methods: We induced dementia related brain damage in organotypic hippocampal slices by -amyloid. Those slices were treated with herb medicines - Hwangryeonhaedoktang, Sopungsoongiwon. Using by PI staining, the extents of cell death were assessed. After that, we selected the best effective one among those herb medicines and the major components of that medicine were studied to reveal neuroprotective effects and related proteins by using PI stating.
Results: In PI staining, Sopungsoongiwon is the best effective herb medicine between Hwangryeonhaedoktang and Sopungsoongiwon. Notopterygii Rhizoma, Corni Fructus, Areca Catechu, Aurantii Fructus Immaturus, Plantaginis Semen is the best effective one among the components of Sopungsoongiwon.
Conclusions: We suggested that purgative effect would be the best effetive medicine on dementia related brain damage between clearing heat and toxic materials.
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KEYWORD
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Notopterygii Rhizoma, Corni Fructus, Areca Catechu, Aurantii Fructus Immaturus, Plantaginis Semen, Neuroprotective, -amyloid, Organotypic hippocampal slice culture
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